The first step to make recombinant DNA is to find restriction enzymes which are enzymes that reconizes a short specific nucleotide sequence in DNA molecules. These enzymes cut those parts so then those sections of DNA are now know as "sticky ends." To make use of recombinat DNA is that it is suppose to replicate and function with the cell. One of the best known ways is to use a plasmid. plasmids are small circular pieces of DNA found in bacteria. The bacteria will reproduce normally but with the DNA you have inserted.
In our experiment we used tetracycline. We chose this because it would kill all the other bacteria except the bacteria with our plasmids. If we used an enzyme that cut the plasmid in two places, then the plasmid would not bind or connect properly with the DNA. This process is important to every day life because with this process you can cure many deceases. We might be able to use this process to change \peoples body parts or increase a specific trait.
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