pGLO Lab

1.

Plate	Number of Colonies	Color of colonies under room light	Color of colonies under   UV light
- pGLO LB	carpet	white	white
- pGLO LB/amp	0	white	white
+ pGLO LB/amp	150~	white	white
+ pGLO LB/amp/ara	70~	white	Green glowing (only 30 glowed)

2.	What two new traits do your transformed bacteria have?
	Resistance to ampicillin and the ability to glow under a UV light.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	There must have been millions but only some bacteria were able to absorb the plasmid therefore killing the rest off.
4.	What is the role of arabinose in the plates?
	Arabinose is the sugar that produces the resistance to ampicillin.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	To tack the motion of other molecules Used to study the Intracellular environment Locating specific cells
	http://www.livescience.com/16752-gfp-protein-fluorescent-nih-nigms.html
6.	Give an example of another application of genetic engineering.

        In medicine, genetic engineering has been used to mass-produce insulin, human growth hormones, follistim (for treating infertility), human albumin, monoclonal antibodies, antihemophilic factors, vaccines, and many other drugs.

Source: Boundless. “Applications of Genetic Engineering.” Boundless Microbiology. Boundless, 21 Jul. 2015. Retrieved 22 Jan. 2016 from https://www.boundless.com/microbiology/textbooks/boundless-microbiology-textbook/microbial-genetics-7/genetic-engineering-products-93/applications-of-genetic-engineering-498-6642/