Unit 6 Reflection

 http://meatcommerce.com/news/eu-debates-farm-animal-cloning-ban-349912

     In this unit the two major things we have learned are bio-ethics and biotech. The basics of bio-ethics is that sometimes some experiments in biology raises some questions or problems concerning morals. For example cloning. People may get angry at these concepts. But it is up to Bio-ethics to resolve these issues or problems to make every one happy. Or at least the majority. But I don't find this to be very interesting in my opinion.

https://pixabay.com/en/pipette-micro-chemistry-test-218135/

 
      Next is Biotech where technology merges with biology. Biotech refers to tools like micro pipettes of microscopes that are used in labs for gel electroscopes.
   
     Some of my strengths were mostly in biotech for I find those topics interesting and enjoyable. I was able to focus and have a great time learning about this topic.
     my weakness, like I said before, is that I do not find this topic very interesting. I would find my self tuning out during lectures and not following along. I ended up on learning that much on Bio-ethics.

     In this unit we did a couple of labs but by far my favorite on was on gel electroscopes. And we used the micro pipette! What I really want to know more about is Biotech and the history behind how it became what it is today. I wounder how people first came up with the idea of biology and how it revolutionized the world.

pGLO Lab

1.

Plate	Number of Colonies	Color of colonies under room light	Color of colonies under   UV light
- pGLO LB	carpet	white	white
- pGLO LB/amp	0	white	white
+ pGLO LB/amp	150~	white	white
+ pGLO LB/amp/ara	70~	white	Green glowing (only 30 glowed)

2.	What two new traits do your transformed bacteria have?
	Resistance to ampicillin and the ability to glow under a UV light.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	There must have been millions but only some bacteria were able to absorb the plasmid therefore killing the rest off.
4.	What is the role of arabinose in the plates?
	Arabinose is the sugar that produces the resistance to ampicillin.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	To tack the motion of other molecules Used to study the Intracellular environment Locating specific cells
	http://www.livescience.com/16752-gfp-protein-fluorescent-nih-nigms.html
6.	Give an example of another application of genetic engineering.

        In medicine, genetic engineering has been used to mass-produce insulin, human growth hormones, follistim (for treating infertility), human albumin, monoclonal antibodies, antihemophilic factors, vaccines, and many other drugs.

Source: Boundless. “Applications of Genetic Engineering.” Boundless Microbiology. Boundless, 21 Jul. 2015. Retrieved 22 Jan. 2016 from https://www.boundless.com/microbiology/textbooks/boundless-microbiology-textbook/microbial-genetics-7/genetic-engineering-products-93/applications-of-genetic-engineering-498-6642/

Candy Electrophoresis Lab

Focus Questions

1.) No, all the dies matched another base dye that we knew.

2.) All these molecules look similar to the dyes and these molecules look like they produce the same dies.

3.) Don't dogs see in different shades of gray? But maybe these different shades of gray appeal to the Dog or Cat.

4.) surprisingly, I do not consume any dyes at all. }I looked at all the food I usually eat and they all said no artificial dyes. Artificial dyes are added to appeal to people and make food look good.

5.) The two factors that control the distance of the colored dyes is the size of the molecules and strength of the electricity.

6.) Electricity moves the dyes through the gel.

7.) The heavy molecules get left behind due to size and weight, and the small dyes move ahead.

8.) The weight of the DNA heavily affects of the distance traveled.  

Recombinat DNA

     The first step to make recombinant DNA is to find restriction enzymes which are enzymes that reconizes a short specific nucleotide sequence in DNA molecules. These enzymes cut those parts so then those sections of DNA are now know as "sticky ends." To make use of recombinat DNA is that it is suppose to replicate and function with the cell. One of the best known ways is to use a plasmid. plasmids are small circular pieces of DNA found in bacteria. The bacteria will reproduce normally but with the DNA you have inserted.
     In our experiment we used tetracycline. We chose this because it would kill all the other bacteria except the bacteria with our plasmids. If we used an enzyme that cut the plasmid in two places, then the plasmid would not bind or connect properly with the DNA. This process is important to every day life because with this process you can cure many deceases. We might be able to use this process to change \peoples body parts or increase a specific trait.